Hide/show bands groups:
D2O : in plane base vibration, sensitive to base pairing and base stacking
H2O : base-sugar, sensitive to glycosidic bond rotation, backbone conformation and sugar puckering
H2O/D2O : sugar-phosphate vibrations, sensitive to backbone conformation
H2O/D2O : sugar vibrations, sensitive to sugar conformations
H-O-H bending mode
H-O-D bending mode
D-O-D bending mode
 
 
Experiment Name dA15-alphadT15Pr_D2O_75
Type DNA
SubtypessDNA
Low Wavenumber 700
High Wavenumber 1800
Interval 0.2
Frequency resolution 1 cm-1
Estimated Strand's concentration 5.74 mM
concentration was estimated based on the Beer-Lambert's law using formula C = OD/(ε x pathlength). ε was estimated with the help of OligoSpec Calculator
Pathlength 5-10 µm
Optical Density 3.5
Solvent D2O
Solvent Comment solution
Added Salt no
Mol. Weight 9137.2
Mol. Weight Ratio 304.57
Cell IdZnSe
UnitsDO,cm-1
Temperature75
Temperature Comment The experiment was carried out around 75°
Noisy very
Sequence Modified yes
Specific Experiment Conditions pH around 7
Expected Structure Unknown
Reference DOI:10.1093/nar/27.21.4151  DOI:10.1080/07391102.2003.10506938 


Sequence

            AAAAAAAAAAAAAAA&亱亱亱亱亱亱亱亱亱亱亱亱亱亱亱
        
Modified residues are showcased with the use of Modomics RNAMods code. "&" symbol means that there are mulitple separate chains.

Unmodifed Sequence

                AAAAAAAAAAAAAAA&亱亱亱亱亱亱亱亱亱亱亱亱亱亱亱
            

Comments

Expected structure is Our interpretation; however, it is possible that there may be mistakes in the interpretation of spectra. In cases where we are uncertain, we have labeled the results as "possible".
The spectra were acquired under specific experimental conditions, meaning the structures are dynamic. As a result, the structures we have obtained may not be the same under different experimental conditions.

Molecular Weight and Molecular Weight Ratio was calculated using OligoCalc.


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