Structural features associated with alpha-anomerie in oligo-α-thymidylates

Overview of Bertrand J.R. et al.

AuthorsBertrand J.R., Bazile D., Malvy C., Paoletti J.
AffiliationUnité de Biochimie, URA 158 CNRS and U 140 INSERM, Institut Gustave-Roussy, 94805-Villejuif, France  
JournalBiochemical and biophysical research communications
Year 1990

Abstract


Comparison between gel electrophoresis migrations of oligo-α-thymidylates and oligo-β-thymidylates indicates that the migration of α-oligonucleotides under native conditions is different from the migration of β-oligonucleotides when the number of thymines, part of the sequence, is higher than 5. Such difference disappears when the gels are run under denaturing conditions. This, together with UV spectra, indicates that the structure of α-oligonucleotides is more organized than the structure of β-oligonucleotides and that such an organisation appears for a length than 5 monomeric units.